Coding

Part:BBa_K3605001:Design

Designed by: Guijie Ren   Group: iGEM20_JNFLS   (2020-10-21)


Glucose hexokinase (GHK) from Gluconacetobacter xylinus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 548
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 478


Design Notes

Some restrict enzymes(such as EcoRI, XbaI, SpeI and PstI) recognition sequence should not be contained.


Source

It comes from the genomic sequenc of Gluconacetobacter xylinus CGMCC 2955.

References